The Trump administration's foreign aid freeze significantly impacted the President's Emergency Plan for AIDS Relief (PEPFAR), a program credited with saving 26 million lives since its inception in 2003. Despite U.S. government directives to continue HIV treatment, testing, and prevention of mother-to-child transmission, a majority of partner organizations still experienced disruptions. According to an AP News report, terminations of awards and delayed payments resulted in the closure of over 1,700 clinics and other service delivery sites, and the loss of more than 16,000 full-time staff members.

Experts from organizations like Health GAP, amfAR, and the International AIDS Society have raised grave concerns about the data, calling it a "five-alarm fire." They point to substantial disruptions across various PEPFAR service areas, marking a "troubling inflection point." While the State Department emphasized that over 20 million people were supported with HIV treatment, only a slight drop from the previous year, critics like Asia Russell argue that for the first time, PEPFAR put fewer people on therapy than the year before, which she considers an "absolute indictment" of the administration.

The cuts led to fewer people being tested for HIV (a 17% drop), fewer newly starting treatment (a 16% decline from 463,000 to 389,000), and a significant hit to PrEP enrollment (a 41% decrease, with new enrollments falling by about 270,000). The direct service-delivery workforce also saw a roughly 24% reduction, with community health workers and case managers being particularly affected. These reductions in frontline workers are linked to a 13% drop in new HIV diagnoses in unaffected clinics and almost a 30% drop in disrupted areas, meaning hundreds of thousands of missing diagnoses and potential for continued HIV spread.

Overall spending on HIV-related activities reportedly declined by 34% in FY25, even though congressional appropriations only fell by about 4% since FY24. Prevention programs were severely impacted, with spending falling by over half and funding for condoms and lubricants being almost completely cut. More than 43% of organizations permanently ceased at least one prevention activity. This shift, heavily focused on treatment at the expense of prevention and support services, risks reversing decades of progress in the global fight against AIDS by 2030, despite the administration's claim that its "America First Global Health Strategy works" by preserving critical care and eliminating wasteful programs.